rabbit polyclonal anti bag1  (R&D Systems)

Journal: Oncology Letters

Article Title: Role of BCL2-associated athanogene in resistance to platinum-based chemotherapy in non-small-cell lung cancer

doi: 10.3892/ol.2015.4003

Figure Lengend Snippet: Correlation between BAG1 chemosensitivity and clinicopathological features of non-small-cell lung cancer patients (n=108).

Article Snippet: A polyclonal rabbit anti-mouse antibody against 3 isoforms of BAG1 [p50 (BAG1L), p46 (BAG1M) and p33 (BAG1S)] (cat. no. sc-939) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques:

Journal: Oncology Letters

Article Title: Role of BCL2-associated athanogene in resistance to platinum-based chemotherapy in non-small-cell lung cancer

doi: 10.3892/ol.2015.4003

Figure Lengend Snippet: (A) Immunohistochemical analysis revealed cytosolic and nuclear expression of BAG1 in lung tissues of NSCLC patients: (a) Cytoplasmic expression of BAG1; (b) cytoplasmic and nuclear expression of BAG1; (c) nuclear expression of BAG1; and (d) negative control (magnification, ×400). (B) Survival curves of patients: (a) time to progression of NSCLC patients with BAG-positive or -negative expression (χ 2 =18.018, P<0.001); and (b) overall survival of NSCLC patients with BAG-positive or -negative expression (χ 2 =24.057, P<0.001). BAG1, BCL2-associated athanogene; NSCLC, non-small-cell lung cancer.

Article Snippet: A polyclonal rabbit anti-mouse antibody against 3 isoforms of BAG1 [p50 (BAG1L), p46 (BAG1M) and p33 (BAG1S)] (cat. no. sc-939) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: Immunohistochemical staining, Expressing, Negative Control

Journal: Oncology Letters

Article Title: Role of BCL2-associated athanogene in resistance to platinum-based chemotherapy in non-small-cell lung cancer

doi: 10.3892/ol.2015.4003

Figure Lengend Snippet: Survival analysis in non-small-cell lung cancer patients by Cox proportional hazards model.

Article Snippet: A polyclonal rabbit anti-mouse antibody against 3 isoforms of BAG1 [p50 (BAG1L), p46 (BAG1M) and p33 (BAG1S)] (cat. no. sc-939) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques:

Journal: Oncology Letters

Article Title: Role of BCL2-associated athanogene in resistance to platinum-based chemotherapy in non-small-cell lung cancer

doi: 10.3892/ol.2015.4003

Figure Lengend Snippet: Tunicamycin-induced endoplasmic reticulum stress in A549 cells. (A) Effects of tunicamycin on the growth of A549 cells. Growth inhibition of A549 cells was examined using MTT assay. Data are presented as the mean ± standard deviation from experiments conducted in triplicate. (B) Impact of tunicamycin on GRP78 and BAG1. A549 cells were treated with 1.25 µg/ml of tunicamycin for 8 h, followed by immunoblot using the indicated antibodies. GRP78, glucose regulated protein 78; BAG1, BCL2-associated athanogene; L, p50 isoform; M, p46 isoform; S, p33 isoform.

Article Snippet: A polyclonal rabbit anti-mouse antibody against 3 isoforms of BAG1 [p50 (BAG1L), p46 (BAG1M) and p33 (BAG1S)] (cat. no. sc-939) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: Inhibition, MTT Assay, Standard Deviation, Western Blot

Journal: Oncology Letters

Article Title: Role of BCL2-associated athanogene in resistance to platinum-based chemotherapy in non-small-cell lung cancer

doi: 10.3892/ol.2015.4003

Figure Lengend Snippet: Impacts of endoplasmic reticulum stress on BAG1 induced by cisplatin treatment. A549 cells were treated with cisplatin in the presence or absence of 1.25 µg/ml tunicamycin. Cell lysates were subjected to immunoblot analysis with indicated antibodies. Tun, tunicamycin; CDDP, cisplatin; BAG1, BCL2-associated athanogene; L, p50 isoform; M, p46 isoform; S, p33 isoform.

Article Snippet: A polyclonal rabbit anti-mouse antibody against 3 isoforms of BAG1 [p50 (BAG1L), p46 (BAG1M) and p33 (BAG1S)] (cat. no. sc-939) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: Western Blot